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Abbkine Inc 40c
40c, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/40c/40c+anti/pm41462543-86-6-23
Average 86 stars, based on 1 article reviews

40c - by Bioz Stars, 2026-09

86/100 stars

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Article Title: Near-infrared photobiomodulation stimulates viability and cartilage matrix synthesis in human chondrocytes
Article Snippet: Cells were permeabilized with 0.1% (w/v) sodium dodecyl sulfate (SDS, Thermo Fisher Scientific, USA) for 10 min at room temperature, followed by blocking with 3% (v/v) bovine serum albumin (BSA, NZYtech, PT) in PBS for 20 min at room temperature. .. Afterwards, chondrocytes were incubated overnight at 40C with primary antibodies diluted in 0.1% BSA (w/v) in phosphate buffered saline (PBS) against COL I (Abbkine, USA, 1:50), COL II (Abbkine, USA, 1:50) and ACAN (Assay Biotechnology, USA, 1:50). .. After three washes with 0.1% BSA (w/v) in PBS, chondrocytes incubated with primary antibody against COL I and COL II were further incubated with anti-mouse secondary antibody conjugated with Andy fluor 488 (ABP Biosciences, USA, 10 μg/mL) in 0.1% BSA (w/v) in PBS, while chondrocytes incubated with primary antibody against ACAN were incubated with anti-rabbit secondary antibody conjugated with Alexa fluor 488 (Abcam, UK, 1:200) in 0.1% BSA (w/v) in PBS for 1 h at room temperature, protected from light.

Article Title: Near-infrared photobiomodulation stimulates viability and cartilage matrix synthesis in human chondrocytes.
Article Snippet: Cells were permeabilized with 0.1% (w/v) sodium dodecyl sulfate (SDS, Thermo Fisher Scientific, USA) for 10 min at room temperature, followed by blocking with 3% (v/v) bovine serum albumin (BSA, NZYtech, PT) in PBS for 20 min at room temperature. .. Afterwards, chondrocytes were incubated overnight at 40C with primary antibodies diluted in 0.1% BSA (w/v) in phosphate buffered saline (PBS) against COL I (Abbkine, USA, 1:50), COL II (Abbkine, USA, 1:50) and ACAN (Assay Biotechnology, USA, 1:50). .. After three washes with 0.1% BSA (w/v) in PBS, chondrocytes incubated with primary antibody against COL I and COL II were further incubated with anti-mouse secondary antibody conjugated with Andy fluor 488 (ABP Biosciences, USA, 10 μg/mL) in 0.1% BSA (w/v) in PBS, while chondrocytes incubated with primary antibody against ACAN were incubated with anti-rabbit secondary antibody conjugated with Alexa fluor 488 (Abcam, UK, 1:200) in 0.1% BSA (w/v) in PBS for 1 h at room temperature, protected from light.

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